SnaBI
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SnaBI Technical Brochure Technical Brochure
SnaBI is a restriction enzyme purified from Sphaerotilus natans.

 

Unit substrate: Lambda DNA (EcoRI digest).

 

Unit calculation assay conditions: 10 mM Bis Tris Propane-HCI (pH 7.0 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37oC.

 

Absence of contaminants: 10 units of SnaBI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA/EcoRI digest at 37oC. After 10-fold overdigestion with SnaBI, greater than 98% of the DNA fragments can be ligated and recut with this enzyme.

 

Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20oC.

 

Heat inactivation: 80oC for 20 minutes.

 

Methylation Sensitivity:

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Blocked

 

Percent Activity in MINOTECH Buffers
 L SH 
 50-75 50  25  <10  100  100 
 
 
General reaction mixture:
10U SnaBI 1μl
10x USnaBI or K buffer*  2μl 
DNA substrate  <1μg
Sterile ultrapure water  Up to 20 μl
Incubate for 15 min at 37oC  
*In the case of USnaBI buffer we recommend the addition of BSA to a final concentration of 100 μg/ml. 

 

Reagents supplied: 10x USnaBI and 10x K buffer