Asu II (isoschizomer) | Technical Brochure | ||||||||||||||||||||||||||||||||||||||
Asu II is a restriction enzyme purified from an isolated strain (#94S).
Unit substrate: Lambda DNA (Hind III digest).
Unit calculation assay conditions: 50 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 0.1% Triton X-100, 100 μg/ml BSA. Incubate at 37oC.
Absence of contaminants: 100 units of AsuII do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNA/Hind III digest at 37oC. After 50-fold overdigestion with AsuII, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.
Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.9@ 25°C), 0.1 mM EDTA, 1 mM dithiothreitol, 0.15% Triton X-100, 200 μg /ml BSA and 50% glycerol. Store at -20oC.
Heat inactivation: 65oC for 20 minutes.
Methylation Sensitivity: dam methylation: Not sensitive dcm methylation: Not sensitive CpG methylation: Blocked
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Reagents supplied: 10x UAsuII and 10x K buffer
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