BglI is a restriction enzyme purified from Bacillus globigii lacking BglII.

Unit substrate: Lambda DNA.

Unit calculation assay conditions: 100 mM KCl, 20 mM Tris-HCl (pH 8.5 @ 25°C), 10 mM MgCl₂, 0.04% Triton X-100, 100 μg/ml BSA. Incubate at 37^oC.

Absence of contaminants: 100 units of BglI do not produce any unspecific  cleavage products after 16 hrs incubation with 1 μg of λ DNA at 37^oC. After 50-fold overdigestion with BglI, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.

Storage buffer: 300 mM NaCl, 20 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM dithiothreitol, 500 μg/ml BSA and 50% glycerol. Store at -20^oC.

Heat inactivation: 65^oC for 20 minutes.

Methylation Sensitivity:

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Blocked by some combinations of overlapping

Reagents supplied: 10x U_BglI and 10x K buffer