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BseA I (BspMII isoschizomer)
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BseA I

(BspMII isoschizomer)

 Technical BrochureTechnical Brochure
 

BseAI is a restriction enzyme purified from Bacillus stearothermophilus.

 

Unit substrate: Lambda DNA.

 

Unit calculation assay conditions: 100 mM NaCl, 10 mM Tris-HCl (pH 8.0 @ 25°C), 5 mM MgCl2, 1 mM dithiothreitol, 0.02% Triton X-100, 100 μg/ml BSA. Incubate at 55oC.

 

Absence of contaminants: 400 units of BseAI do not produce any unspecific  cleavage products after 16 hrs incubation with 1 μg of λ DNA at 55oC. After 100-fold overdigestion with BseAI, greater than 98% of the DNA fragments can be ligated and recut with this enzyme.

 

 

Storage buffer: 50 mM KCl, 10 mM  Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 500 μg/ml BSA and 50% glycerol. Store at -20oC.

 

 

Heat inactivation: No.

 

 

Reference: Thanos, D., Scarpelis, G., Papamatheakis, J. and Bouriotis, V. (1989). Nucleic Acids Res. 17, 8881.

 

Percent Activity in MINOTECH Buffers
 L M  H  SH  A  K 
 10 50 75-100 50-75 10 100
 
 
General reaction mixture:
10U BseA I 1μl
10x UBseAI or K buffer*  2μl 
DNA substrate  <1μg
Sterile ultrapure water  Up to 20 μl
Incubate for 15 min at 55oC  
*In the case of UBseAI buffer we recommend the addition of
BSA to a final concentration of 100 μg/ml. 

 

Reagents supplied: 10x UBseAI and 10x K buffer