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BseB I (BstN I isoschizomer)
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BseB I (BstN I isoschizomer)
Technical BrochureTechnical Brochure
 

BseBI is a restriction enzyme purified from Bacillus stearothermophilus.

 

Unit substrate: Lambda DNA.

 

Unit calculation assay conditions: 50 mM NaCl, 10 mM Tris-HCl (pH @ 7.9 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 60°C.

 

Absence of contaminants:  500 units of BseBI do not produce any unspecific  cleavage products after 16 hrs incubation with 1 μg of λ DNA at 60oC. After ten-fold overdigestion with BseBI, less than 50% of the DNA fragments can be ligated.

 

Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20oC.

 

Heat inactivation: No.

 

Note: BseBI-cut DNA is difficult to ligate with T4 DNA Ligase. Ligation is enhanced in the presence of 15% PEG4000.

 

Percent Activity in MINOTECH Buffers
 L M  H  SH  A  K 
 10-25 100 50 25-50 <10 50
 
 
General reaction mixture:
10U BseB I 1μl
10x M buffer*  2μl 
DNA substrate  <1μg
Sterile ultrapure water  Up to 20 μl
Incubate for 15 min at 60oC  

*We recommend the addition of BSA to a final
concentration of 100 μg/ml.

Reagents supplied: 10x M buffer