Large scale (mg to gram) protein purification of tagged or untagged proteins. Our team is highly-experienced in downstream processing steps and can help you optimize your protein purification scheme. A series of different types of chromatography can be tested to your protein (size exclusion, Ion exchange, affinity, hydrophobic interaction). The PFF’s, FPLC equipments are capable of up to 100ml/min flow rates.
What we offer:
Large scale growth (20 or 30lt) of your bacterial strain and upscale-optimization of the purification scheme.
Please contact us for more information.
The FF focuses on controlled production of biomass (mainly microbial) over a wide range of controlled conditions (culture media, temperature, pH, aeration, stirring, etc). The FF houses all equipment needed for the maintenance, growth, collection and storage of microorganisms (bacteria, fungi, etc). Cell mass is produced according to strict quality criteria and is accompanied with Specifications and Technical data sheet that includes all relevant information on growth conditions.
What we offer:
Optimization of your protein expression or biomass growth and upscale to our bioreactors. You just bring us a plate with your desired bacterial strain and we deliver you cell pellet (from 20lt or 30lt culture).
Please contact us for more information.
Our production team is preparing a database with all E.coli cloning and expression strains and plasmids that IMBB research teams have in their collection. Our R&D team is willing to collaborate with other research teams that need support in heterologous expression using E.coli as the Trojan horse.
You can watch our presentation on “Heterologous Expression in E.coli” in order to get a few tips when designing your own experiment.