* Size:

XbaI Technical Brochure Technical Brochure

XbaI is a restriction enzyme purified from Xanthomonas badrii.


Unit substrate: Lambda DNA (dam-/HindIII digest).


Unit calculation assay conditions: 50 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37oC.


Absence of contaminants: 200 units of XbaI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNAdam-/HindIII digest at 37oC. After 100-fold overdigestion with XbaI, greater than 98% of the DNA fragments can be ligated and recut with this enzyme.


Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol , 500 μg/ml BSA and 50% glycerol. Store at -20oC.


Heat inactivation: 65oC for 20 minutes.


Methylation Sensitivity:

dam methylation: Blocked by overlapping

dcm methylation: Not sensitive

CpG methylation: Not sensitive 


Percent Activity in MINOTECH Buffers
 L SH 
 50-75 100  75  75  75  100 
General reaction mixture:
10U XbaI 1μl
10x M or K buffer*  2μl 
DNA substrate  <1μg
Sterile ultrapure water  Up to 20 μl
Incubate for 15 min at 37oC  
*In the case of M buffer we recommend the addition of BSA to a final concentration of 100 μg/ml. 


Reagents supplied: 10x M and 10x K buffer