XbaI is a restriction enzyme purified from Xanthomonas badrii.

Unit substrate: Lambda DNA (dam^-/HindIII digest).

Unit calculation assay conditions: 50 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl₂, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37^oC.

Absence of contaminants: 200 units of XbaI do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of λ DNAdam^-/HindIII digest at 37^oC. After 100-fold overdigestion with XbaI, greater than 98% of the DNA fragments can be ligated and recut with this enzyme.

Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol , 500 μg/ml BSA and 50% glycerol. Store at -20^oC.

Heat inactivation: 65^oC for 20 minutes.

Methylation Sensitivity:

dam methylation: Blocked by overlapping

dcm methylation: Not sensitive

Reagents supplied: 10x M and 10x K buffer