Lambda DNA PstI Digest
(concentration 0.5μg/μl)

Description: The Pst I digest of λ DNA yields the following discrete fragments (in base pairs): 11501*, 5077, 4749, 4507, 2838, 2556*, 2459, 2443, 2140, 1986, 1700, 1159 1093, 805, 514, 468, 448, 339, 264, 247, 216, 211, 200, 164, 150, 94, 87, 72, 15.

Preparation: Lambda DNA was completely digested by Pst I, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.

Storage buffer:  10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Store at -20^oC.

Note: Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH₂O and subsequently heated.

*The cohesive ends (12b cos site of bacteriophage λ) of fragments 11501bp and 2556bp may anneal to and form the additional band. These fragments may be separated by heating to 65^oC for 5 min and then cooling on ice for 3min.

Related products: 10X Loading Dye Solution