Taq DNA Polymerase
* Size:

Taq DNA Polymerase
(Concentration 5 u/μl)

Technical Brochure

Source: Purified from an E. coli strain carrying a plasmid with Taq DNA polymerase gene from Thermus aquaticus YT-1.


Description: Taq DNA Polymerase is a thermostable enzyme that catalyzes 5'3' synthesis of DNA. The enzyme has no detectable 3'5' proofreading exonuclease activity, but possesses low 5'3' exonuclease activity.

Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmoles of dNTPs into acid insoluble material in 30 minutes at 72oC.


Reaction conditions: 1x Taq polymerase buffer [50 mM KCl, 10 mM Tris-HCl pH 8.5 @ 25oC, 1.5 mM MgCl2, 0.1% Triton X-100].


Quality Control Assays:

  •     Standard DNA Polymerase Assay Conditions (not PCR conditions): The polymerase activity is assayed in 10 mM Tris-HCl (pH 8.5), 50 mM KCl, 1.5 mM MgCl2, 200 μM each of dATP, dGTP, dCTP, dTTP (a mix of unlabeled and [3H] dTTP) and 12.5μg activated calf thymus DNA, in a final volume of 50 μl.


  • Functional Assay: Taq DNA Polymerase is tested for performance in the polymerase chain reaction (PCR) using 1.5 units of enzyme to amplify a 1730-bp region of the PspP I methyltransferase gene from 5 ng of bacterial genomic DNA. The resulting PCR product is visualized as a single band on an ethidium bromide-stained agarose gel.


  • Absence of contaminants: Tested extensively for the absence of endo- and exodeoxyribonucleases.

Guaranteed stability: Taq DNA polymerase is guaranteed to maintain stability for six months from the date of shipment when stored as directed.

Storage Buffer: 100 mM NaCl, 50 mM Tris-HCl (pH 8.0 @ 25oC), 1 mM DTT, 0.1 mM EDTA, 1% Triton X-100 and 50%  glycerol. Store at -20oC.

Recommended PCR mixture: 
10x Taq pol. buf.  5 μl
10mM dNTP mix  1 μl
25μΜ forward primer  1 μl
25μΜ reverse primer  1 μl
Template DNA  1-500 ng
Taq DNA pol. (5 u/μl)  0.25-0.5 μl
Sterile ultrapure water  Up to 50 μl
Recommended PCR conditions:
Initial denaturation  94oC, 2min


PCR Cycles 
Denature  94oC, 45sec
Anneal*  45-68oC, 30sec
Extend  72oC, 1min/kb
Final extension  72oC, 10min
Hold  4oC, indefinitely
*Anneal temperature depends on primer Tm 



Reagents supplied: 10x Taq DNA polymerase Buffer (1.5ml) and/or 10x Taq DNA polymerase w/o MgCl2 (1.5ml) and 25mM MgCl2 (1.5ml).