StyI is a restriction enzyme purified from E.coli WA921/pST27 hsd+.

Unit substrate: Lambda DNA.

Unit calculation assay conditions: 100 mM NaCl, 50 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl₂, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37^oC.

Absence of contaminants: 50 units of StyI do no produce any unspecific clevage products after 16 hrs incubation with 1 μg of λ DNA at 37°C. After 50-fold overdigestion with StyI, greater than 98% of the DNA fragments can be ligated and recut with this enzyme.

Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20^oC.

Heat inactivation: 65^oC for 20 minutes.

Methylation Sensitivity:

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

Reagents supplied: 10x H buffer