* Size:

StyI Technical Brochure Technical Brochure

StyI is a restriction enzyme purified from E.coli WA921/pST27 hsd+.


Unit substrate: Lambda DNA.


Unit calculation assay conditions: 100 mM NaCl, 50 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml BSA. Incubate at 37oC.


Absence of contaminants: 50 units of StyI do no produce any unspecific clevage products after 16 hrs incubation with 1 μg of λ DNA at 37°C. After 50-fold overdigestion with StyI, greater than 98% of the DNA fragments can be ligated and recut with this enzyme.


Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml BSA and 50% glycerol. Store at -20oC.


Heat inactivation: 65oC for 20 minutes.


Methylation Sensitivity:

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive


Star activity: Conditions of low ionic strength, high enzyme concentration, glycerol concentration >5%, or pH>8.0 may result in star activity 


Percent Activity in MINOTECH Buffers
 L SH 
 25-50 75-100  100  75-100  <10  50 
General reaction mixture:
10U StyI 1μl
10x H buffer*  2μl 
DNA substrate  <1μg
Sterile ultrapure water  Up to 20 μl
Incubate for 15 min at 37oC  
*We recommend the addition of BSA to a final concentration of 100 μg/ml. 


Reagents supplied: 10x H buffer