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Semi-prestained Protein Marker
Size:


Semi-prestained Protein Marker
(usage 5μl/lane)

 Technical BrochureTechnical Brochure
Description: The semiprestained Protein Marker is a mixture of 8 highly purified unstained proteins and 3 prestained proteins (16, 34, 85 kDa) that resolve into 11 identifiable bands from 14.4-85 kDa when analysed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250, or the protein stain of choice. This marker is intended for use as a precise size standard when performing SDS-PAGE in order to calculate the molecular weight of a protein of your interest and as marker for western blot experiments.

 

Preparation: Gently mix the semiprestained Protein Marker by vortexing or pipetting up and down several times. Pipette the desired amount (5-7μl/lane) to a separate tube. Incubate at 95-100oC for 5 minutes, spin down and load.

 

Storage buffer:  62,5mM Tris-HCl (pH=6,8), 2% SDS, 1mM EDTA, 30% Glycerol, 0,01% Bromophenol blue, 1mM NaN3, 50mM DTT. Store at -20oC.

 

Note:

  • Do not boil the aliquots a second time.
  • The recommended load is 5-7μl in a gel lane. Loading less amount may result in less intense bands.
  • Run gel using constant voltage, according to manufacturer’s specifications
  • Run until dye reaches the bottom of the gel, but does not run off

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