|
pUC19 DNA |
 Technical Brochure |
| Description: pUC19 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases. The molecular weight of pUC19 is 1.75x106 daltons.
Preparation: pUC19 is isolated from E. coli strain HB101 by a standard plasmid purification procedure.
Quality control: Gel analysis for purity. BsiSI and AluI fragmentation patterns.
Storage buffer: 10 mM Tris-HCl (pH 8.0), and 1 mM EDTA. Store at -20oC.
Related products: 10X Loading Dye Solution |
 |
