Lambda DNA StyI Digest
* Size:

Lambda DNA StyI Digest
(concentration 0.5μg/μl)

Technical BrochureTechnical Brochure
Description: The Sty I digest of λ DNA yields the following 11 discrete fragments (in base pairs): 19329*, 7743, 6223, 4254*, 3472, 2690, 1882, 1489, 925, 421, 74.


Preparation: Lambda DNA was completely digested by Sty I, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.


Storage buffer:  10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Store at -20oC.


Note: Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.


*The cohesive ends (12b cos site of bacteriophage λ) of fragments 19329bp and 4254bp may anneal to and form the additional band. These fragments may be separated by heating to 65oC for 5 min and then cooling on ice for 3min.