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Lambda DNA EcoR I/Hind III Digest
* Size:



Lambda DNA EcoRI/HindIII Digest
(concentration 0.5μg/μl)

  Technical Brochure
Description: The EcoR I and Hind III digest of λ DNA yields the following 13 discrete fragments (in base pairs): 21226*, 5148, 4973, 4268, 3530*, 2027, 1904, 1584, 1375, 947, 831, 564, 125.

 

Preparation: Lambda DNA was completely digested by EcoR I and Hind III, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM  EDTA.

 

Storage buffer:  10 mM Tris-HCl (pH 8.0) and 1 mM  EDTA. Store at -20oC.

 

Note: Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.

 

*The cohesive ends (12b cos site of bacteriophage λ) of fragments 21226bp and 3530bp may anneal to and form the additional band. These fragments may be separated by heating to 65oC for 5 min and then cooling on ice for 3min.

 

 

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