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Lambda DNA BstE II Digest |
 Technical Brochure |
| Description: The BstE II digest of λ DNA yields the following 14 discrete fragments (in base pairs):
8453*, 7242, 6369, 5687*, 4822, 4324, 3675, 2323, 1929, 1371, 1264, 702, 224, 117.
Preparation: Lambda DNA was completely digested by BstE II, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
Storage buffer: 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Store at -20oC.
Note: Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated.
*The cohesive ends (12b cos site of bacteriophage λ) of fragments 8453bp and 5687bp may anneal to and form an additional band of 14140bp. These fragments may be separated by heating to 65oC for 5 min and then cooling on ice for 3min.
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