Hinf I
* Size:

Hinf I Technical BrochureTechnical Brochure

HinfI is a restriction enzyme purified from a recombinant E.coli strain.


Unit substrate: Lambda DNA.


Unit calculation assay conditions: 100 mM NaCl, 50 mM Tris-HCl (pH 7.9 @ 25oC), 10 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml bovine serum albumin and DNA. Incubate at 37oC.


Absence of contaminants: 200 units of Hinf I do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of Lambda DNA at 37oC. After 100-fold overdigestion with Hinf I, greater than 90% of the DNA fragments can be ligated and recut with this enzyme.


Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml bovine serum albumin and 50% glycerol. Store at -20oC.


Heat inactivation: 80oC for 20 minutes.


Methylation Sensitivity:

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Blocked by some combinations of overlapping


Percent Activity in MINOTECH Buffers
 L SH 
10-25  50  100  75-100 50  100 
General reaction mixture:
10U HinfI 1μl
10x H or K buffer *  2μl 
DNA substrate  <1μg
Sterile ultrapure water  Up to 20 μl
Incubate for 15 min at 37oC  
*In the case of H buffer we recommend the addition of BSA to a final concentration of 100 μg/ml.


Reagents supplied: 10x H and 10x K buffer