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BssAI (Cfr10 I isoschizomer)
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BssAI (Cfr10 I isoschizomer) Technical BrochureTechnical Brochure
 

BssAI is a restriction enzyme purified from Bacillus species.

 

Unit substrate: Lambda DNA.

 

Unit calculation assay conditions: 100 mM KCl, 20 mM Tris-HCl (pH 8.5 @ 25oC), 3 mM MgCl2, 0.04% Triton X-100, 100 μg/ml bovine serum albumin and DNA. Incubate at 65oC.

 

Absence of contaminants: 50 units of BssA I do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of Lambda DNA at 65oC. After 30-fold overdigestion with BssA I, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.

 

Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml bovine serum albumin and 50% glycerol. Store at -20oC.

 

Heat inactivation: No.

 

Methylation Sensitivity:

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

 

Reference: Rina, M., Stratidakis, I. and Bouriotis, V. (1990). Nucleic Acids Res. 18, 6161.

 

Percent Activity in MINOTECH Buffers
 L M  H  SH  A  K 
 10 25 75 50 25 100
 
 
General reaction mixture:
10U BssAI
1μl
10x UBssAI or K buffer*  2μl 
DNA substrate  <1μg
Sterile ultrapure water  Up to 20 μl
Incubate for 15 min at 65oC  
*In the case of UBssAI buffer we recommend the addition of BSA
to a final concentration of 100 μg/ml. 

 

Reagents supplied: 10x UBssAI and 10x K buffer