Sca I
* Size:




Sca I Technical Brochure Technical Brochure
ScaI is a restriction enzyme purified from Streptomyces caespitosus.

 

Unit substrate: Lambda DNA.

 

Unit calculation assay conditions: 100 mM KCl, 10 mM Bis-Tris-Propane (pH 7.0 @ 25°C), 10 mM MgCl2, 100 μg/ml BSA. Incubate at 37°C.

 

Absence of contaminants: 600 units of Sca I incubated for 16 hours at 37ºC with 1 μg of λ DNA resulted in a DNA pattern free of detectable nuclease degradation as determined by agarose gel electrophoresis. After 100-fold overdigestion with Sca I, greater than 90% of the DNA fragments can be ligated and recut with this enzyme.

 

Storage buffer: 50 mM KCl, 10 mM Tris-HCl  (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 500 μg/ml BSA and 50% glycerol. Store at -20oC.

 

Heat inactivation: 80oC for 20 minutes.

 

Methylation Sensitivity:

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

 

Star activity: Large excess of the enzyme may results in the appearance of star activity.

 

Percent Activity in MINOTECH Buffers
 L SH 
 <10 50-75  100  75-100 25 50 
 
 
General reaction mixture:
10U Sca I 1μl
10x UScaI buffer*  2μl 
DNA substrate  <1μg
Sterile ultrapure water  Up to 20 μl
Incubate for 15 min at 37oC  
*We recommend the addition of BSA to a final concentration of 100 μg/ml. 

 

Reagents supplied: 10x UScaI buffer