pBR322 DNA
* Size:



pBR322 DNA
(concentration 0.5μg/μl)

Technical Brochure Technical Brochure
Description: pBR322 is a commonly used plasmid cloning vector in E. coli. The molecule is double-stranded circle, 4361 base pairs in length. pBR322 contains the genes for resistance to ampicillin and tetracycline, and can be amplified with chloramphenicol. The molecular weight of pBR322 is 2.83x106 daltons.

 

Preparation: pBR322 is isolated from E. coli strain HB101 by a standard plasmid purification procedure.

 

Quality control: Gel analysis for purity. HinfI and AluI fragmentation patterns.

 

Storage buffer: 10 mM Tris-HCl (pH 8.0), and 1 mM EDTA. Store at -20oC.

 

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