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Lambda DNA Hind III Digest |
 Technical Brochure |
| Description: The Hind III digest of λ DNA yields the following 8 discrete fragments (in base pairs): 23130*, 9416, 6557, 4361*, 2322, 2027, 564, 125.
Preparation: Lambda DNA was completely digested by Hind III, phenol extracted, ethanol precipitated, dissolved in 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
Storage buffer: 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA. Store at -20oC.
Note: Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O and subsequently heated..
*The cohesive ends (12b cos site of bacteriophage λ) of fragments 23130 and 4361 may be separated by heating to 65oC for 5 minutes.
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