BsiS I (Hpa II isoschizomer)
* Size:




BsiS I (Hpa II isoschizomer) Technical BrochureTechnical Brochure
 

BsiSI is a restriction enzyme purified from Bacillus stearothermophilus.

 

Unit substrate: Lambda DNA.

 

Unit calculation assay conditions: 66 mM potassium acetate, 33 mM Tris-acetate (pH 7.9 @ 25oC), 10 mM magnesium acetate, 0.5 mM dithiothreitol, 0.1% Triton X-100, 100 μg/ml bovine serum albumin and DNA. Incubate at 55oC.

 

Absence of contaminants: 150 units of BsiS I do not produce any unspecific cleavage products after 16 hrs incubation with 1 μg of Lambda DNA at 55oC. After 50-fold overdigestion with BsiS I, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.

 

Storage buffer: 50 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1mM dithiothreitol, 200 μg/ml bovine serum albumin and 50% glycerol. Store at -20oC.

 

Heat inactivation: No.

 

Methylation Sensitivity:

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

 

Reference: Rina, M. and Bouriotis, V. (1990) Nucleic Acids Res: 18, 1654

 

Percent Activity in MINOTECH Buffers
 L SH 
 25 50 25 10-25 100 100
 
 
General reaction mixture:
10U BsiSI 1μl
10x UBsiSI or K buffer*  2μl 
DNA substrate  <1μg
Sterile ultrapure water  Up to 20 μl
Incubate for 15 min at 55oC  
*In the case of UBsiSI buffer we recommend the addition of BSA
to a final concentration of 100 μg/ml.
 

 

Reagents supplied: 10x UBsiSI and 10x K buffer