Bgl I
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Bgl I
Technical BrochureTechnical Brochure
 

BglI is a restriction enzyme purified from Bacillus globigii lacking BglII.

 

Unit substrate: Lambda DNA.

 

Unit calculation assay conditions: 100 mM KCl, 20 mM Tris-HCl (pH 8.5 @ 25°C), 10 mM MgCl2, 0.04% Triton X-100, 100 μg/ml BSA. Incubate at 37oC.

 

Absence of contaminants: 100 units of BglI do not produce any unspecific  cleavage products after 16 hrs incubation with 1 μg of λ DNA at 37oC. After 50-fold overdigestion with BglI, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.

 

Storage buffer: 300 mM NaCl, 20 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 1 mM dithiothreitol, 500 μg/ml BSA and 50% glycerol. Store at -20oC.

 

Heat inactivation: 65oC for 20 minutes.

 

Methylation Sensitivity:

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Blocked by some combinations of overlapping

Percent Activity in MINOTECH Buffers
 L SH 
 10-25 75-100 75-100 75-100 50 100
 
 
General reaction mixture:
10U  1μl
10x UBglI or K buffer * 2μl 
DNA substrate  <1μg
Sterile ultrapure water  Up to 20 μl
Incubate for 15 min at 37oC  
*In the case of UBglI buffer we recommend the addition of
BSA to a final concentration of 100 μg/ml.
 

Reagents supplied: 10x UBglI and 10x K buffer