AsuII (isoschizomer)
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Asu II (isoschizomer) Technical BrochureTechnical Brochure
 

Asu II is a restriction enzyme purified from an isolated strain (#94S).

 

Unit substrate: Lambda DNA (Hind III digest).

 

Unit calculation assay conditions: 50 mM NaCl, 10 mM Tris-HCl (pH 7.9 @ 25°C), 10 mM MgCl2, 1 mM dithiothreitol, 0.1% Triton X-100, 100 μg/ml BSA. Incubate at 37oC.

 

Absence of contaminants:  100 units of AsuII do not produce any unspecific  cleavage products after 16 hrs incubation with 1 μg of λ DNA/Hind III digest at 37oC.  After 50-fold overdigestion with AsuII, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.

 

Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.9@ 25°C), 0.1 mM EDTA, 1 mM dithiothreitol, 0.15% Triton X-100, 200 μg /ml BSA and 50% glycerol. Store at -20oC.

 

Heat inactivation: 65oC for 20 minutes.

 

Methylation Sensitivity:

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Blocked

Percent Activity in MINOTECH Buffers
L M H SH A K
75 100 50-75  25  50  100
 

 

General reaction mixture:
10U AsuII  1μl
10x UAsuII or K buffer *  2μl
DNA substrate  <1μg
Sterile ultrapure water  Up to 20 μl
Incubate for 15 min at 37oC  

*In the case of UAsuII buffer we recommend the addition of
BSA to a final concentration of 100 μg/ml.

 

 

Reagents supplied: 10x UAsuII and 10x K buffer