AluI
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AluI
Technical BrochureTechnical Brochure

AluI is a restriction enzyme purified from Arthrobacter luteus (ATCC 21606).

 

Unit substrate: Lambda DNA.

 

Unit calculation assay conditions: 10 mM Tris-HCl (pH 7.9 @ 25oC), 10 mM MgCl2, 1 mM dithiothreitol, 100 μg/ml bovine serum albumin and DNA. Incubate at 37oC.

 

Absence of contaminants:  50 units of AluI do not produce any unspecific cleavage products  after 16 hrs incubation with 1 μg of lambda DNA at 37oC. After 10-fold overdigestion with Alu I, greater than 95% of the DNA fragments can be ligated and recut with this enzyme.

 

Storage buffer: 100 mM KCl, 10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM dithiothreitol, 200 μg/ml bovine serum albumin and 50% glycerol.Store at -20oC.

 

Heat inactivation: 65oC for 20 minutes.

 

Methylation Sensitivity:

dam methylation: Not sensitive

dcm methylation: Not sensitive

CpG methylation: Not sensitive

 

Percent Activity in MINOTECH Buffers
L  M  H  SH A  K
 100  100 75  10-25 75 100
 
 

General reaction mixture:

 
10U AluI  1μl
10x L or K buffer *  2μl
DNA substrate <1μg
Sterile ultrapure water  Up to 20 μl
 

*In the case of L buffer we recommend the addition of
BSA to a final concentration of 100 μg/ml.

 Reagents supplied: 10x L and 10x K buffer